Immunohistochemical study of Epidermal Growth Factor Receptor (EGFR), Hepatocyte Growth Factor (HGF), c-MET, Vascular Endothelial Growth Factor (VEGF) and CD34 in non small cell carcinomas of the lung Abbreviated title: Immunohistochemical study of NSCLC

Immunohistochemical study of Epidermal Growth Factor Receptor (EGFR), Hepatocyte Growth Factor (HGF), c-MET, Vascular Endothelial Growth Factor (VEGF) and CD34 in non small cell carcinomas of the lung Abbreviated title: Immunohistochemical study of NSCLC

Maria Sakalidou, Dimitrios Bouklas, Georgia-Heleni Thomopoulou, Sophia Tseleni, Andreas C. Lazaris, Nikolaos Kavantzas



Aim: The aim of our study was the immunohistochemical investigation of the expression of Epidermal Growth Factor Receptor (EGFR), Hepatocyte Growth Factor (HGF), c-MET and Vascular Endothelial Growth Factor (VEGF) in non small cell lung cancer (NSCLC) tissue samples.

Materials and Methods: Forty seven cases of NSCLC were selected from the archive material of the First Department of Pathology of National and Kapodistrian University of Athens. The immunohistochemical staining was performed using anti- EGFR/ HGF/c-MET/VEGF/ CD 34 antibodies. The immunoexpression was correlated with angiogenesis events, using the CD34 marker, and a qualitative and quantitative evaluation was performed. All data were statistically correlated with each other, so as with various clinicopathologic parameters τοο, in an attempt to draw conclusions about their prognostic significance.

Results: VEGF expression was found to be higher in squamous cell carcinomas and was negatively associated with the expression of CD34 and HGF. The last two were positively correlated with higher tumor grades and disease stage. CD34 and HGF expression was found to be higher in adenocarcinomas. Lastly, CD34 and HGF expression was found to be associated with lymph node infiltration.


The expression of the selected markers varies among different histological types of NSCLC.

Tumors with a positive expression of CD34 are more aggressive (high grade).

Tumors with positive expression of angiogenic factors are diagnosed at an advanced stage of the disease.



Epidermal Growth Factor Receptor, Hepatocyte Growth Factor/c-MET, Vascular Endothelial Growth Factor, CD34, non small cell lung cancer

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